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Technical Articles
Tech Article
Complete Workflow for DUB Inhibitor Screening: Protocol and Critical Quality Controls
Establishing a robust DUB inhibitor screening system requires rigorous quality control. This article details the complete workflow from compound preparation and enzyme reaction setup to data analysis, with acceptable ranges for critical QC parameters.
Tech Article
Ub-AMC vs Ub-Rho110: A Practical Guide to Fluorogenic Substrate Selection
Ub-AMC and Ub-Rho110 are the two most widely used fluorogenic substrates for DUB activity assays. This article provides a four-dimension comparison — sensitivity, wavelength, cost, and application — to help researchers select the optimal substrate for their experimental needs.
Tech Article
DUB Assay Technology — Principles and Application Guide
Deubiquitinases (DUBs) play key regulatory roles in the ubiquitin-proteasome system. This article introduces the principles of fluorogenic substrate-based DUB activity assays and key experimental design considerations.